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Click on any filter to expand it and set it.

Filters, even if unselected, tell you just how your search results are distributed across various aspects of an interaction. The number of interactions that match a particular filter item with respect to the current query are displayed in parenthesis beside each item. As you refine your search these numbers will change to reflect the other filters selected and/or your search terms.

An interaction can be 'multi-valued' with respect to a filter. For example an interaction can be supported by evidence from several source databases, so it contributes to the match count of each those databases.

• • Source Database

    bind 62648

    bind_translation 60369

    biogrid 286327

    corum 2607

    dip 72248

    hprd 40515

    innatedb 5495

    intact 199895

    matrixdb 228

    mpact 13338

    mpidb 1206

    mppi 778

    ophid 47432

    Can match ANY of these Must match ALL of these Must match NONE of these
     

    seen by 1 DB 367196

    seen by 2 DBs 76415

    seen by 3 or more DBs 66265
  • Organism **

    single organism interaction 470147

    cross organism interaction 39729
     

    Homo sapiens 222465

    Saccharomyces cerevisiae S288c 117322

    Drosophila melanogaster 45587

    Mus musculus 30263

    Arabidopsis thaliana 21469

    Escherichia coli K-12 15326

    Caenorhabditis elegans 14124

    Campylobacter jejuni subsp. jejuni NCTC 11168 11974

    Schizosaccharomyces pombe 972h- 8626

    Rattus norvegicus 8291

    Can match ANY of these Must match ALL of these Must match NONE of these
  • Nature of Interaction

    unary 18539

    pairwise 470798

    multi-subunit 20539
     

    predicted 47435

    experimental 485444
     

    genetic 96

    physical 509825
• • MI (MINT-Inspired) Score

    0 or more 386271

    0.1 or more 386264

    0.2 or more 325443

    0.3 or more 242794

    0.4 or more 214090

    0.5 or more 141267

    0.6 or more 113964

    0.7 or more 92054

    0.8 or more 71027

    0.9 or more 53001

    Can match ANY of these Must match ALL of these Must match NONE of these

    MI (MINT Inspired) scores are in the range [0,1], are limited to interactions where all its proteins are the same species, supported by valid PubMed IDs, not predicted or from OPHID, not a self-interaction, and are from one of the following organisms:

    • A. thaliana
    • B. taurus
    • C. elegans
    • D. melanogaster
    • H. sapiens

    • M. musculus
    • O. sativa
    • R. norvegicus
    • S. cerevisiae
    • S. pombe

    Any interaction that does not meet the above requirements has no score calculated and this is displayed as 'n/c'

    Go here to find out how the score is calculated.

  • MI (MINT-Inspired) Organism Percentile

    0 or more 386271

    10 or more 372575

    20 or more 338243

    30 or more 328758

    40 or more 248642

    50 or more 210627

    60 or more 168845

    70 or more 131207

    80 or more 92714

    90 or more 39339

    Can match ANY of these Must match ALL of these Must match NONE of these
• • Interaction Detection Method *

    3d-structure 2

    DNase I footprinting 537

    Identification by mass spectrometry 2

    Split renilla luciferase complementation 20841

    acetylase assay 1

    acetylation reaction 345

    adenylate cyclase complementation 5032

    adp ribosylase assay 3

    affinity chromatography technology 5

    affinity technology 4

    amplified luminescent proximity homogeneous assay 4

    anti bait coimmunoprecipitation 6

    anti tag coimmunoprecipitation 1

    anti tag immunostaining 424

    anti tag western blot 283

    antibody array 15

    antisense rna 2

    array technology 32599

    atomic force microscopy 17

    autoradiography 467

    bead aggregation assay 115

    beta galactosidase complementation 2

    beta lactamase complementation 5

    bimolecular fluorescence complementation 21610

    bio-layer interferometry 3

    biochemical 1913

    bioluminescence resonance energy transfer 8

    biophysical 25027

    biosensor 1887

    biotin tag 1016

    blue native page 10

    carboxylation assay 9

    chromatin immunoprecipitation array 17

    chromatin immunoprecipitation assay 4

    chromatography technology 1

    circular dichroism 95

    classical fluorescence spectroscopy 132

    cleavage assay 4

    cleavage reaction 15786

    coimmunoprecipitation 5

    colocalization 4

    colocalization by fluorescent probes cloning 3

    colocalization/visualisation technologies 1

    comigration in gel electrophoresis 8932

    comigration in non denaturing gel electrophoresis 1348

    comigration in sds page 251

    competition binding 57

    confirmation by molecular weight 1

    confirmational text mining 7352

    confocal microscopy 236

    copurification 17

    cosedimentation 7

    cosedimentation in solution 684

    cosedimentation through density gradient 6856

    cytoplasmic complementation assay 328

    de novo protein sequencing by mass spectrometry 47

    deacetylase assay 20

    deaminase assay 1136

    decarboxylation assay 47619

    deglycosylase assay 47432

    demethylase assay 10558

    dephosphorylation reaction 2379

    detection by mass spectrometry 23

    deubiquitinase assay 1796

    deubiquitination reaction 5

    dihydrofolate reductase reconstruction 43

    display technology 42

    dna directed dna polymerase assay 1

    docking 2

    dynamic light scattering 3

    edman degradation 2823

    electron diffraction 449

    electron microscopy 8298

    electron paramagnetic resonance 17

    electron resonance 15

    electron tomography 6754

    electrophoretic mobility shift assay 1

    electrophoretic mobility supershift assay 37

    electrophoretic mobility-based method 4255

    enhancement interaction 2

    enzymatic footprinting 5

    enzymatic reaction 18

    enzymatic study 38442

    enzyme linked immunosorbent assay 12

    enzyme-mediated activation of radical sources 1

    equilibrium dialysis 28

    experimental knowledge based 8

    experimental participant identification 19589

    far western blotting 7

    field flow fractionation 2

    filamentous phage display 1

    filter binding 35

    filter trap assay 222

    fluorescence correlation spectroscopy 1

    fluorescence microscopy 217

    fluorescence technology 121978

    fluorescence-activated cell sorting 35

    fluorescent resonance energy transfer 3

    footprinting 20

    full identification by DNA sequencing 119

    gal4 vp16 complementation 43

    gdp/gtp exchange assay 21

    genetic 4

    genetic interference 305

    glycosylase assay 1

    gst pull down 3

    gtpase assay 463

    his pull down 20

    histone acetylase assay 128

    homogeneous time resolved fluorescence 267

    identification by antibody 1307

    imaging technique 62

    immunocytochemistry 12

    immunodepleted coimmunoprecipitation 16

    immunohistochemistry 3

    immunostaining 15

    in gel phosphatase assay 6

    in vitro 26

    in vivo 1113

    in-gel kinase assay 6

    inferred by author 1874

    inferred by curator 9492

    inhibitor small molecules 3

    interaction detection method 4

    interaction prediction 63

    interactome parallel affinity capture 193

    intermolecular force 108

    interologs mapping 1

    ion exchange chromatography 63

    isothermal titration calorimetry 247

    isotope label footprinting 12

    kinase scintillation proximity assay 94

    lambda phage display 11

    lambda repressor two hybrid 279

    lex-a dimerization assay 658

    light microscopy 1

    light scattering 47

    liposome binding assay 64

    luminescence based mammalian interactome mapping 32

    mammalian protein protein interaction trap 37

    mass spectrometry studies of complexes 6416

    mass spectrometry study of hydrogen/deuterium exchange 59393

    membrane bound complementation assay 2

    methyltransferase assay 298

    methyltransferase radiometric assay 175

    microscale thermophoresis 588

    miniaturized immunoprecipitation 19

    mobility shift 492

    molecular sieving 427

    molecular weight estimation by autoradiography 1

    molecular weight estimation by coomasie staining 1925

    molecular weight estimation by silver staining 2

    molecular weight estimation by sybr staining 4

    monoclonal antibody blockade 1

    monoclonal antibody immunostaining 6

    monoclonal antibody western blot 191

    multidimensional protein identification technology 65

    mutation analysis 61

    neutron diffraction 709

    northern blot 240

    nuclear magnetic resonance 521

    nucleic acid uv cross-linking assay 58

    nucleotide sequence identification 321

    one hybrid 2

    p3 filamentous phage display 1

    partial DNA sequence identification by hybridization 96

    partial identification of protein sequence 89785

    peptide array 1

    peptide massfingerprinting 444

    peptide synthesis 14

    phage display 53495

    phenotype-based detection assay 1

    phosphatase assay 392

    phosphotransferase assay 9887

    polyclonal antibody immunostaining 2

    polyclonal antibody western blot 2698

    polymerization 2

    post transcriptional interference 133

    predetermined feature 8

    predetermined participant 15

    predictive text mining 1

    primer specific pcr 7

    protease accessibility laddering 4

    protease assay 28449

    protein complementation assay 2

    protein cross-linking with a bifunctional reagent 165

    protein folding/unfolding 204

    protein phosphatase assay 27

    protein sequence identification 1

    protein three hybrid 5

    proximity ligation assay 1289

    pull down 29

    quantitative pcr 122

    reverse phase chromatography 941

    reverse ras recruitment system 46

    reverse two hybrid 2236

    ribonuclease assay 178

    rna interference 448

    sandwich immunoassay 1875

    saturation binding 1

    scanning electron microscopy 2

    scintillation proximity assay 56

    sequence tag identification 2

    silicon nanowire field-effect transistor 31

    small angle neutron scattering 672

    solid phase assay 3

    solution state nmr 168

    southern blot 1

    spin label 1

    split firefly luciferase complementation 7

    static light scattering 170687

    structure based prediction 5

    sumoylation reaction 1

    surface plasmon resonance 429

    surface plasmon resonance array 8846

    synthetic 43

    synthetic genetic analysis 5

    systematic evolution of ligands by exponential enrichment 1150

    t7 phage display 12605

    tag visualisation 403

    tag visualisation by enzyme assay 187

    tag visualisation by luciferase assay 1

    tag visualisation by peroxidase activity 25

    tandem affinity purification 196

    tap tag coimmunoprecipitation 43

    text mining 16

    three hybrid 38

    tox-r dimerization assay 424

    transcriptional complementation assay 12

    transmission electron microscopy 6

    two hybrid array 7896

    two hybrid fragment pooling approach 7

    two hybrid pooling approach 1

    two hybrid prey pooling approach 20565

    ubiquitin binding 2

    ubiquitin reconstruction 157

    ubiquitinase assay 5

    ubiquitination reaction 1136

    unknown 66128

    unspecified method 339

    x ray scattering 168

    x-ray crystallography 2811

    yeast display 819

    zymography 1

    Can match ANY of these Must match ALL of these Must match NONE of these
  • Interaction Type *

    acetylation reaction 39

    adp ribosylation reaction 58

    aggregation 47432

    amidation reaction 1

    association 41043

    cleavage reaction 119

    colocalization 21564

    covalent binding 212

    deacetylation reaction 30

    deformylation reaction 1

    demethylation reaction 7

    deneddylation reaction 1

    dephosphorylation reaction 377

    deubiquitination reaction 10

    direct interaction 134082

    disulfide bond 37

    dna strand elongation 1

    enzymatic reaction 86

    genetic interaction 96

    glycosylation reaction 2

    gtpase reaction 2

    hydroxylation reaction 8

    methylation reaction 58

    molecular interaction 120185

    neddylation reaction 4

    oxidoreductase activity electron transfer reaction 16

    palmitoylation reaction 6

    phosphorylation reaction 1675

    phosphotransfer reaction 18

    physical association 329283

    protein cleavage 94

    putative self interaction 2

    rna cleavage 1

    self interaction 6

    sumoylation reaction 7

    transglutamination reaction 3

    ubiquitination reaction 73

    Can match ANY of these Must match ALL of these Must match NONE of these
• • Number of Publications

    no valid PubMed ID 0

    1 or more publications 507210

    2 or more publications 76572

    3 or more publications 30394

    4 or more publications 17521

    Can match ANY of these Must match ALL of these Must match NONE of these
  * Filter counts for interaction method and type propagate to their parent terms as defined by the PSI-MI ontology.
  Thus a selected method or type filter will return all of its child terms as well.
  ** Organism filter counts (for instance yeast and its subspecies) do not propagate.

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